Bioanalytical Characterization and Production
Consultative oriented services for the isolation, analysis, characterization, and production of proteins and peptides. Our breath of services and experience in rational design can support the entire array of discovery and development needs for drug and diagnostic development organizations, from discovery to market. GLP and CLIA compliance with BSL-3 facilities.
View a printable PDF of this page
Please select a technology to find out more about our protein & peptide chemistry services:
- Proteomics
- Peptide Synthesis and Mapping
- Biochemical Analysis
- Amino Acid Composition
- Biophysical Characterization and Ligand Binding
- Protein Sequence Analysis
- Recombinant Protein Expression
- Antibody Production
2-D gel electrophoresis and proteome analysis from gels
Protein isolation, purification, and characterization from gels
Informatics by UniProt, PIR, GenEmbl
[return to top]
Milligram to hundred gram range solid phase, solution phase
Fully automated and manual approaches
Peptide libraries
Peptide modifications: Radiolabel, chromogenic, fluorogenic, stable isotope, non-natural amino acids
Peptide mapping
RP HPLCC
MALDITOF/MS
LC/ES/MS
[return to top]
Chromatographic mapping
Mass spectral analysis and mapping by MALDI-TOF/MS, ES/MS, or PSD/CID/CAF
Mass spectral protein sequencing
2D-gels
SDS-Page with and without reduction
FPLC SEC for apparent mol. Wt.
Light Scattering for determination of aggregates
[return to top]
Quantitative determination of all free occurring amino acids
Determination of free amino acids
Unusual amino acids: HyPro, HyLys
Physiological amino acids
Solution phase, PVDF membrane, PAGE gel samples
[return to top]
Biophysical Characterization and Ligand Binding
Protein and ligand binding studies by isothermal titration calorimetry (ITC).
Determination the binding constant and stoichiometry of binding between molecules, as well as the molar heat, free energy, and entropy of binding.
Protein and peptide thermal stability characterization by differential scanning calorimetry (DSC)
Thermal stability, determination of melting points, temperature and molar heat of unfolding, interpretations can be made as to the mechanism of unfolding.
Protein structural analysis by Circular Dichroism, Florescence Spectroscopy, Protein Florescence, Light Scattering Spectroscopy (SEC)
[return to top]
N-terminal sequence Analysis
Automated Edman degradation
Solution phase, PVDF, PAGE gel or resin-bound peptide samples
C-terminal Sequence Analysis
Enzymatic degradation using carboxypeptidase Y
Five time points for analysis
[return to top]
Recombinant Protein Expression
[return to top]
Antibody Production
[return to top]