Expression, Purification, and Characterization
Starting with sequence data, CBI can design over-expression systems, and generate purified recombinant proteins for biopharmaceutical development and diagnostic applications. CBI can support drug and diagnostic development organizations from discovery to market. GLP and CLIA compliance with BSL-3 facilities.
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Please select a technology to find out more about our recombinant proteins services:
- Protein Target Development
- Expression Systems
- Purification of recombinant proteins
- Characterization of recombinant proteins
- Biophysical Characterization
Custom gene synthesis and codon optimization
PCR amplification of encoding sequence from genomic or plasmid DNA
Proteins can be designed with "HIS-TAGS", "GST-TAGS", or client selected
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Selection of appropriate expression vector and insertion of target sequence
Transfection of host cell lines including bacterial, yeast, mammalian, and insect cells
Pichia expression
Small scale fermentation to assess expression levels and characterize protein
Cell lines include
Mammalian
Bacterial
Yeast
Insect
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Purification of Recombinant Proteins
Development of purification protocols
Cell lysis and precipitation
Chromatography and polishing chromatography
Purification to > 95%
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Characterization of Recombinant Proteins
Protein sequencing and mass spectrometry
Mass spectral analysis and mapping my MALDI-TOF/MS, ES/MS, PDS/CID/CAF
ELISA
SDS-Page and Western Blots
Amino acid analysis
Stability Testing
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Isothermal titration calorimetry (ITC). Determination the binding constant and stoichiometry of binding between molecules, as well as other thermodynamic parameters such as the molar heat, free energy, and entropy of binding.
Thermal stability characterization by differential scanning calorimetry (DSC)
Structural analysis by Circular Dichroism, Florescence Spectroscopy, Protein Florescence, Light Scattering Spectroscopy (SEC)
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Scale Up Fermentation
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