Antibody Production and ELISA Development
Starting with immunogen or epitope sequence information, through purification and characterization of a therapeutic or diagnostic antibody, CBI can support drug and diagnostic development organizations from discovery to market. GLP and CLIA compliance with BSL-3 facilities.
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Please select a technology to find out more about our immunology services:
- Immunogen / Epitope Preparation and Characterization
- Monoclonal Antibodies
- Polyclonal Antisera
- Monospecific Polyclonal Antisera
- Antibody Characterization
- Labeled Antibodies
- ELISA Development
Immunogen / Epitope Preparation and Characterization
Immunogen / Epitope Preparation and CharacterizationEpitope analysis and prediction of immunogenic peptide sequences
MAPS (4 or 8 arm peptide sequences)
Fully automated and manual preparation of immunogenic peptides
Conjugation of immunogens to carrier proteins
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Hybridoma development
Immunization and screening
Fusion and screening of hybridomas
Cloning and ascites productions
In Vivo monoclonal production
In Vitro monoclonal production
Purification of IgGs, IgMs, IgYs
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Immunization with supplied material or immunogens prepared by CBI
Purification by large scale protein A or L, differential precipitation, DEAE chromatography or gel filtration chromatography
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Monospecific Polyclonal Antisera
Immobilization of antigen to column matrix or synthesized to antigen synthesized directly to column resin de novo
Purification by peptide affinity ligand chromatography
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Antibody Structural Characterization
Analysis with intact IgG and subsequent mass spectral analysis and amino acid analysis
Chain separation by SDS-Page
Direct N-terminal sequence analysis of separated heavy chain and light chain
Peptide mapping of heavy and light chains by chromatographic or mass spectral analysis
Primary sequence determination by digestion and mass spectroscopy
Ligand binding studies by isothermal titration calorimetry (ITC)
Thermal stability by differential scanning calorimetry (DSC)
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Custom labeling with biotin, HRP, AP, FITC, etc.
ELISA determination of titers
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Direct, indirect, and sandwich ELISA protocols
Ligand supplied or developed at CBI
Assay validation protocols
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