Antibody Services

Antibody Structure Characterization

CBI has implemented a specific program for characterization of antibody structure which conforms to the following format

1. Studies with the intact IgG
   • Mass spectral analysis to determine molecular weight
   • b. Complete amino acid analysis to determine protein content and residue equivalents per mol. This consists of 5 separate hydrolyses for quantitation of all residues, including Cys, Cystine, and Trp
2. Chain separation by SDS PAGE
   • a. Elution of the separated chains from the SDS PAGE gel
3. Direct N-terminal sequence analysis of the separated Heavy Chain (10 cycles):
4. Direct N-terminal sequence analysis of the separated Light Chain (10 cycles):
   • If necessary, use of pyro-Gly aminopeptidase or Deactylase to deblock the N-terminus:
5. C-terminal sequence analysis of the separated Heavy Chain
   • C-terminal sequence analysis of the separated Light Chain
6. Peptide mapping of the separated heavy chain.
7. Peptide mapping of the separated light chain.
8. Mass spec and/or automated Edman degradation primary sequence determination of individual peptide fragments from the digest of the separated heavy chain.
9. Mass spec and/or automated Edman degradation primary sequence determination of individual peptide fragments from the digest of the separated light chain.

Request a Quotation

Commonwealth Biotechnologies, Inc. 601 Biotech Drive, Richmond, VA 23235 800-735-9224 (800-RELY-CBI) FAX: 804-648-2641 email: [email protected]