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Protein ID by Mass SpecCBI offers full service mass spec capabilities, including all aspects of proteomics (MALDI or Q-TOF instrumentation). We routinely perform ES/MS, LC/ES/MS or MALDI-TOF/MS analyses of proteins peptides, oligonucleotides, and various other organic (and non-organic) molecules. Acquisitions can be done in the negative or positive ion modes, and coupled with LC, mass species in the range of 50-150,000 Da (albeit quite often, as multiply charged ions) can be visualized with pmol (or lower) sensitivities. The amount of material needed for an experiment is typically on the order of 10 pmol, but clearly if sample manipulation is required (sep pak, dialysis, etc) prior to the mass spec experiment, we will need more material to allow for losses during sample handling.
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