Amino Acid Analysis

Amino acid analysis is typically done by acid (vapor or solution phase) or base hydrolysis of protein or peptide samples, and we also offer short time (2 hour) analyses in propionic acid/HCl. Quantitative determination of all residues (including Trp, Pro, and Hypro) is possible at the 800 fmol level, but the routine dynamic range is 10-100 pmoles.

In a single time point acid hydrolysis, all residues except Cys and Trp can be quantitated. However, to fully quantitate Ser and Thr, a second, longer time point of hydrolysis is required.

CBI offers reduction and alkylation (with iodoacetamide, iodoacetic acid, or 4-vinyl pyridine) of samples for determination of Cys; free Cys can be determined following alkylation only, and total Cys content following reduction and alkylation. Trp is determined on yet another aliquot of sample following hydrolysis in methanesulfonic acid. Each of these methods is considered a separate analysis, and bears a separate cost.

Amino Acid Prices

Amino acid analysis is $75 per analysis.