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CBI will design your protein for overexpression in E. coli, yeast, or baculoviral systems. Proteins can be designed with “his-tags”, “GST-tags”, or other system of your choice. CBI recommends a regulatable expression system to provide optimum protein yields, but will work with the vector system of your selection. In our process, overexpressed proteins are:
• Analyzed by SDS-PAGE
• Purified using the affinity tags or other chromatographic step
• Verified by N-terminal sequence analysis, if requested
Standard proteins are provided at ~85% purity. Additional purification steps are available if needed.
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Commonwealth Biotechnologies, Inc. 601 Biotech Drive, Richmond, VA 23235 800-735-9224 (800-RELY-CBI) FAX: 804-648-2641 email: [email protected] |
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