Oligonucleotide Synthesis

DNA/RNA Oligonucleotide Synthesis

CBI offers DNA synthesis on a 50nmol, 0.2, 1, or 15 µmol scale. 50nmol level DNA synthesis is ideal for sequencing primers, PCR primers, and adapters or linkers for subcloning projects. 1 µmole synthesis is suitable for antisense DNA or repetitive PCR. Purification by RPC, HPLC, or PAGE is also available. CBI also specializes in synthesis of modified oligonucleotides, e.g., 5' phosphate-on, biotinylated, phosphorothioate, flourescent, etc.

Coupling efficiencies exceed 98% and the crude oligo can usually be used directly for PCR or DNA sequencing. For standard oligo synthesis, the turnaround time is 24-48 hours, quality control is performed on each sample, and a gel photo is provided.

CBI is known as a world leader in high quality, custom RNA synthesis. We have specialized over the past 6 years in modified RNA synthesis and have dedicated a portion of our synthesis facility to RNA synthesis exclusively.

For RNA, we offer 1 µmol-scale syntheses. Turnaround time is approximately 5 to 7 working days, and quality control is performed on each oligo. Purification of RNA is provided by HPLC or PAGE, and a quality control gel photo is provided. Larger scale synthesis is also available (please inquire). Chimeric DNA/RNA and modified RNA oligonucleotide syntheses are available.

Experienced CBI professionals are available to help you define your synthesis requirements.



Nucleic Acid Synthesis Form (Excel Version)
Nucleic Acid Synthesis Order Form (Open Office Version)
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Commonwealth Biotechnologies, Inc. 601 Biotech Drive, Richmond, VA 23235 800-735-9224 (800-RELY-CBI) FAX: 804-648-2641 email: [email protected]