Immunology

Antibody Production and ELISA Development

Starting with immunogen or epitope sequence information, through purification and characterization of a therapeutic or diagnostic antibody, CBI can support drug and diagnostic development organizations from discovery to market. GLP and CLIA compliance with BSL-3 facilities.

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Please select a technology to find out more about our Immunology services:

• Immunogen / Epitope Preparation and Characterization
• Monoclonal Antibodies
• Polyclonal Antisera
• Monospecific Polyclonal Antisera
• Antibody Characterization
• Labeled Antibodies
• ELISA Development

Immunogen / Epitope Preparation and Characterization Epitope analysis and prediction of immunogenic peptide sequences MAPS (4 or 8 arm peptide sequences) Fully automated and manual preparation of immunogenic peptides Conjugation of immunogens to carrier proteins [return to top]

Monoclonal Antibodies Hybridoma development Immunization and screening Fusion and screening of hybridomas Cloning and ascites productions In Vivo monoclonal production In Vitro monoclonal production Purification of IgGs, IgMs, IgYs [return to top]

Polyclonal Antisera Immunization with supplied material or immunogens prepared by CBI Purification by large scale protein A or L, differential precipitation, DEAE chromatography or gel filtration chromatography [return to top]

Monospecific Polyclonal Antisera Immobilization of antigen to column matrix or synthesized to antigen synthesized directly to column resin de novo Purification by peptide affinity ligand chromatography [return to top]

Antibody Structural Characterization Analysis with intact IgG and subsequent mass spectral analysis and amino acid analysis Chain separation by SDS-Page Direct N-terminal sequence analysis of separated heavy chain and light chain Peptide mapping of heavy and light chains by chromatographic or mass spectral analysis Primary sequence determination by digestion and mass spectroscopy Ligand binding studies by isothermal titration calorimetry (ITC) Thermal stability by differential scanning calorimetry (DSC) [return to top]

Labeled Antibodies Custom labeling with biotin, HRP, AP, FITC, etc. ELISA determination of titers [return to top]

ELISA Development Direct, indirect, and sandwich ELISA protocols Ligand supplied or developed at CBI Assay validation protocols [return to top]

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