Commonwealth
Biotechnologies, Inc.
911 E. Leigh St.
Richmond, VA 23219
tel: (800) 735-9224
(800) RELY-CBI
fax: (804) 648-2641
e-mail: [email protected]
----serving the biomedical research community since 1992----
Protein Sequence Analysis
CBI offers two protein sequence protocols: N-terminal analysis by automated Edman degradation, and C-terminal analysis by carboxypeptidase digestion. A brief description and the prices for these services are given below:
N-terminal Analysis by automated Edman Degradation:
Starting with as little of 100 pmol of material, up to 25 residues are routinely unambiguously assigned. Quantitation of all residues is possible at the 5-10 pmol level. Many samples are provided as transblots of proteins on polyvinylidene difluoride (PVDF) membranes. With about 30 pmol of transblotted sample, as many as 15-20 residues can usually be assigned. New technologies permit direct analysis of individual protein bands excised from SDS-PAGE gels. CBI provides a transblotting and gel elution service for a an additional cost if requested. Direct sequencing of synthetic peptides still bouind to their synthesis resin is also possible.
C-terminal Analysis by Carboxypeptidase Digestion:
Starting with 1-2 nmol of protein substrate, 5-7 time point aliquots are taken, normally providing assignment of 7-10 residues. The protein sample can be provided as a solid, or in a volatile buffer such as N-morpholine acetate, pH 8.5, or 30 mM ammonium bicarbonate, pH 8.5. The concentration of the protein is critical for this technique.
Prices:
N-terminal Sequence Analysis:
First Cycle, baseline and standards: $90.00
Subsequent Cycles: $55.00
C-terminal Sequence Analysis:
Carboxypeptidase digestion: $300.00
Amino acid analysis: $40.00 per time point; target protein
Amino acid analysis: $40.00 per time point; autolysis blank
Please call or e-mail for additional information.
Click
Home Page to return to the CBI Home Page.
For questions and information relevant to this Web Site, contact
Webmaster-CBI.