| The Centers for Disease Control and Prevention (CDC) and the
Association of Public Health Laboratories (APHL) have worked together to
develop the Laboratory Response Network for the express purpose of dealing with
bioterrorism and other terrorism threats.
Our laboratory at CBI, following
the protocols developed by the CDC, APHL, and the Laboratory Response Network,
offers testing services for suspect samples or environmental monitoring. All
samples which test positive at CBI will be forwarded to the appropriate public
health laboratories, appropriate government agencies or law enforcement
laboratories for confirmation.
CBI utilizes the facilities and expertise it has developed over the past ten
years to provide the critical analysis necessary to identify bacteria in
suspect environmental samples. Through many years of contract government work,
CBI has advanced the knowledge and understanding of detection in complex
samples.
Powders
Lab testing of powders or other materials suspected of harboring the anthrax
bacteria can be performed. Positive tests will be reported and the sample
passed on to public safety laboratories or Federal government laboratories for
confirmatory analysis.
Tests include:
- Culture of suspected materials.
- Microscopic examination of a sample of a suspect material, Gram Stain.
- Evaluation of the growth properties in broth
- PCR (polymerase chain reaction) test that amplifies DNA at three sites.
- Microbiological analysis, Hemolysis, AP ID testing, and morphology
analysis.
- Other specialized tests to confirm the identification of the bacteria.
Environment (where exposure may have occurred)
During the course of an investigation important information may be obtained
from sampling the environment where the exposure may have occurred. Testing of
the environment is useful for detecting trace amounts of anthrax spores.
Specimens obtained may include:
- Swabs on contamination of various surfaces
- Samples of the air (off filters)
- Paper samples
- Soil samples
- Cloth
These samples are processed in the laboratory to promote growth of any
spores, if present. If suspicious bacteria grow, additional testing may be
performed.
Monitoring
CBI experts can work with you to devise continual monitoring of your
facility or place of business for detection of anthrax or other agents. CBI's
monitoring program can include post-decontamination surface testing and
viability testing of decontaminated materials.
Background information on Bioterrorism and Anthrax
- CDC. Ongoing
investigation of anthrax---Florida, October 2001. MMWR 2001;50:877
- CDC. Human
Anthrax Associated With an Epizootic Among Livestock---North Dakota, 2000. MMWR
2001;50:677--80.
- NATURE: Overview of scientific literature on Anthrax
http://www.nature.com/nature/anthrax
- Thomas Inglesby, et. al. JAMA, Vol. 281, No.18, May 1999.
Anthrax
as a Biological Weapon.
- CDC
guidelines for sample collection and monitoring.
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