Commonwealth Biotechnologies, Inc.

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Commonwealth Biotechnologies has recently developed a new DNA sequencing loading buffer (AccuTrac ®) which provides a marking tool for sample tracking in Automated DNA sequencing applications. Specifically, use of AccuTrac® as the sample loading buffer run on an automated DNA sequencer produces a "mark" on the gel at the beginning of each individual lane into which a sample was loaded. Marking the lane aids in identification of individual lanes during the manual review of the tracking process and ensures that the instrument accurately applies data to the appropriate sample.

AccuTrac® is detected by an automated sequencer without interfering with the data generated by the instrument. The reagent incorporates a fluorescent dye which is recognized by most, if not all, currently available DNA sequencers. The formulation of AccuTrac® is PROPRIETARY to CBI.

Below is a gel file picture of AccuTrac ® run on a PE-Applied Biosystems 377 XL sequencer. The DNA samples were prepared by ABI PRISM¨ BigDye® Terminators cycle sequencing kits and post sequencing clean up was performed by using Edge Biosystems spin columns.

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The dye band at the bottom of gel enables accurate lane identification without interfering with the data contained in the lane. Below is shown a pgem control sequenced using AccuTrac in the loading buffer. The data shows a "GG" base call (AccuTrac® marker) at the start of sequence data but does not obstruct any "real data".

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AccuTrac® has been used in thousands of reactions, for both BigDye® and FS dye-terminator reactions. It has also been used in concert with a variety of different clean up protocols and instrumentation. Please inquire if you have any questions regarding application of AccuTrac to your specific chemistry and instrumentation.

A second marker, AccuTrac-P®, has been developed for those using dye primer chemistry and/or ethanol preparation. Of course, custom products may be available for your application, please inquire.

To purchase either AccuTrac® product, please call (800) 735-9224 ext. 144. If you prefer, you may receive a faxed order form by calling the CBI Toll-free Fax on Demand line at 877-FAX-4CBI (877-329-4224). Select option 4 for order forms. You may also order faxed information on any of CBI's services from our Fax on Demand line.

Try our ONLINE ORDER form to order AccuTrac® or AccuTrac-P ®.

Commonwealth Biotechnologies, Inc.
601 Biotech Drive, Richmond, VA 23235
800-735-9224 (800-RELY-CBI) FAX: 804-648-2641
email: [email protected]